Pharmaceutical Microbiology – I Important Questions with Answers , Unit Wise Guide (B pharma 3rd semester solved)
Section A
Unit-I Questions
- Differentiate between Pharmaceutical and Medical Microbiology.
- Give the difference between Prokaryotic and Eukaryotic cells.
- Give the contribution of Louis Pasteur in the field of Microbiology.
- Enlist types of Microscopy.
- Admit all the physical parameters required for bacterial growth.
- How will you define Pasteurization?
- Draw a neat labeled diagram of a bacterial cell.
Unit-II Questions
- Enlist the different staining techniques.
- Write any four names of equipment for sterilization.
- Define Mechanical Sterilization with an example.
- Comment on the correlation between D-value and Z-value in sterilization.
- Explain the DOP test of HEPA filters.
- Define Sterilization.
- What do you understand by IMViC?
- Enlist different types of phenol coefficient tests.
Unit-III Questions
- Define antiseptic and disinfectant.
- What is disinfectant? Provide two examples.
- Differentiate bactericidal and bacteriostatic.
- What is the difference between yeast and moulds?
- Classification and mode of action of disinfectants (implied from syllabus)
- What is microbial spoilage?
- Define microbial contamination.
- Give the difference between antiseptic and disinfectant with examples.
- Write an annotated note on the microbiological assay of amino acids.
Unit-IV Questions
- Define aseptic area.
- Enumerate the sources of contamination in the aseptic area.
- Elucidate clean area classification according to ISO.
- What do you understand by aseptic and clean areas?
- Define microbial assay with two examples.
- Explain the discovery of Penicillin.
- What is the contribution of Alexander Fleming in Microbiology?
Unit-V Questions
- Differentiate microbial spoilage of pharmaceutical products.
- Write the applications of cell culture.
- How will you define cell culture?
- Enlist the factors affecting the microbial spoilage of pharmaceutical products.
- Explicate primary cell culture.
Section B
Unit-I Questions
- Write in detail about the scope and application of pharmaceutical microbiology.
- Give the morphological classification of bacteria with suitable examples and diagrams.
- Explain the ultra-structure of bacteria and give its brief morphological classification.
Unit-II Questions
- Write the principle, procedure, and application of Ziehl-Neelsen staining.
- Write the mechanism, procedure, and advantages of Gram staining technique.
- Expound the physical methods of sterilization with principle, procedure, and applications.
Unit-III Questions
- Write the classification and mode of action of disinfectants.
Unit-IV Questions
- Enumerate the parameters required for designing an aseptic area.
- Describe the method for microbial standardization of Vitamin B12 or streptomycin.
Unit-V Questions
- Discuss the applications of cell culture in pharmaceuticals and research.
- Discuss various types of animal cell cultures and write the procedure for isolation of cells for in-vitro culture.
Section C
Unit-I Questions
- Explain the ultra-structure and morphological classification of bacteria.
- Write the principle, application, advantages, and disadvantages of phase contrast microscopy.
- Describe the bacterial growth curve with all phases.
- Describe various factors affecting bacterial growth.
Unit-II Questions
- Differentiate between moist heat and dry heat sterilization.
- Give the principle, working, and application of a moist heat sterilizer.
- Describe different types of sterility indicators for the evaluation of sterilization.
- Write about Gram staining in detail with its principle and procedure.
Unit-III Questions
- What are the factors influencing disinfection?
- Define virus. Give methods of cultivation of viruses in detail.
- Illustrate methods for sterility testing of pharmaceutical products according to IP.
- Explain the reproductive cycles of viruses.
- Explain microbial assay of erythromycin.
Unit-IV Questions
- Explain different sources of contamination in an aseptic area and methods of prevention.
- Describe the concept and design of clean and aseptic areas.
- Discuss the methods for prevention of contamination in aseptic areas.
- Elaborate on different methods used for the microbiological assay of antibiotics or vitamins.
- What are the various air flow patterns in laminar flow equipment?
Unit-V Questions
- Explain cell cultures in the pharmaceutical industry and research.
- Discuss the factors affecting microbial spoilage of pharmaceutical products.
- How to preserve pharmaceutical products with the help of antimicrobial agents?
- Why do we need to preserve microbes? Explain any two methods for preservation.